Ecodistribution, infection rates and host preference of tsetse flies in the sleeping sickness focus of Bonon, west-central Côte d’Ivoire
DOI:
https://doi.org/10.17159/2254-8854/2025/a20242Keywords:
Glossina, Trypanosoma, apparent density per trap, blood meal, human African trypanosomiasisAbstract
The sleeping sickness focus of Bonon was the last one still active at a low endemic level in Côte d’Ivoire. An entomological survey carried out in June 2015 during the rainy season using “Vavoua” traps guided subsequent control activities. Indeed, it improved knowledge of tsetse fly ecology. All the tsetse flies caught (i.e. 1909) belonged to the subspecies Glossina palpalis palpalis (Robineau-Desvoidy, 1830), the major vector of Human African Trypanosomiasis (HAT) in Côte d’Ivoire. In this paper, we looked at the relationship between the apparent density (AD, flies/trap/day) and biotopes. The AD significantly varied according to biotopes, with high density around villages. The trypanosomes overall infection rate (mature and immature) according to microscopic observation was 23.2%. When considering mature infections, the infection rate was 5.5 %. Polymerase chain reaction (PCR) analyses confirmed the presence of Trypanosoma brucei s.l. and Trypanosoma congolense “forest type”. Blood meals analysis using cytochrome b gene sequences revealed that tsetse flies fed on pigs. The edges of the villages seem to constitute preferred habitats for tsetse flies where they are protected from insecticide pressure in the fields, and where they can easily take bloodmeals from free-ranging pigs. The findings of this study provided a baseline in decision-making for subsequent vector control activities.
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References
Acapovi-Yao G, Allou K, Mavoungou J, Zoh D, Dia M, N’goran KE. 2013. Distribution géographique et infection de Glossina palpalis palpalis (Diptera, Glossinidae) par les trypanosomes dans des reliques forestières de la ville d’Abidjan-Côte d’Ivoire. Revue Africaine de Santé et de Productions Animales. 11:37–42.
Allou K, Acapovi-Yao G, Kaba D, Bosson-Vanga H, Solano P, N’Goran KE. 2009. Chorologie et infection par les trypanosomes de Glossina palpalis palpalis dans la forêt du Banco et ses reliques, Abidjan (Côte d’Ivoire). Parasite. 16(4):289–295. https://doi.org/10.1051/parasite/2009164289. DOI: https://doi.org/10.1051/parasite/2009164289
Bouyer J, Desquesnes M, Yoni W, Chamisa A, Guerrini L. 2015. Attracting and trapping insect vectors. Technical Guide No. 2, 22 p.
Bosson-Vanga A, Acapovi-Yao G, Kaba D, Dofini F, Coulibaly B, N’Dri L, Koné M. 2012. Infection de Glossina palpalis palpalis par les trypanosomes le long du fleuve Comoé dans la région d’Abengourou (Côte d’Ivoire). Journal of Pharmaceutical and Biological Sciences. 13:31–37.
Boulangé A, Lejon V, Berthier D, Thévenon S, Gimonneau G, Desquesnes M, Abah S, Agboho P, Chilongo K, Gebre T, et al. 2022. The COMBAT project: controlling and progressively minimizing the burden of vector-borne animal trypanosomosis in Africa. Open Research Europe. 2(67):67. https://doi.org/10.12688/openreseurope.14759.2. DOI: https://doi.org/10.12688/openreseurope.14759.1
Büscher P, Cecchi G, Jamonneau V, Priotto G. 2017. Human African trypanosomiasis. Lancet. 390(10110):2397–2409. https://doi.org/10.1016/S0140-6736(17)31510-6. DOI: https://doi.org/10.1016/S0140-6736(17)31510-6
Challier A, Gouteux JP. 1980. Ecology and epidemiological importance of Glossina palpalis in the Ivory Coast forest zone. International Journal of Tropical Insect Science. 1(1):77–83. https://doi.org/10.1017/S1742758400000187. DOI: https://doi.org/10.1017/S1742758400000187
Challier A, Gouteux JP, Coosemans M. 1983. La limite géographique entre les sous-espèces Glossina palpalis palpalis (Rob.-Desv.) et G. palpalis gambiensis Vanderplank (Diptera : Glossinidae) en Afrique occidentale. Cahiers ORSTOM. Série Entomologie Médicale et Parasitologie. 21(4): 207–220.
Cecchi G, Paone M, de Gier J, Zhao W. 2024. The continental atlas of the distribution of tsetse flies in Africa. PAAT Technical and Scientific Series, No. 12. Rome: FAO. https://doi.org/10.4060/cd2022en. DOI: https://doi.org/10.4060/cd2022en
Coulibaly B, Krouba DI, Kouakou AAC, Ouattara AA, Berté D, Ta BTD, Rayaisse JB, Jamonneau V, Solano P, Koffi YJJ, et al. 2018. Conséquences sanitaires de la dynamique du paysage rural dans le foyer de Trypanosomiase Humaine Africaine (THA) de Bonon (Côte d’Ivoire) entre 2002 et 2015. Revue Espace Territoires Sociétés et Santé. 1(2):19–36.
Courtin F, Dupont S, Zézé DG, Jamonneau V, Sané B, Coulibaly B, Cuny G, Solano P. 2005. Trypanosomose Humaine Africaine : transmission urbaine dans le foyer de Bonon (Cote d’Ivoire). Tropical Medicine & International Health. 10(4):340–346. https://doi.org/10.1111/j.1365-3156.2005.01398.x. DOI: https://doi.org/10.1111/j.1365-3156.2005.01398.x
Djè NN, Miezan TW, N’Guessan P, Brika P, Doua F, Boa F. 2002. Distribution géographique des trypanosomés pris en charge en Côte d’Ivoire de 1993 à 2000. Bulletin de la Sociét é de Pathologie Exotique. 95:359–361.
Dagnogo M, Lohuirignon K, Gouteux J-P. 1985. Comportement alimentaire des populations péridomestiques de Glossina palpalis (Robineau – Desvoidy) et de Glossina tachinoides Westwood du domaine guinéen de Côte d’Ivoire. Cahiers ORSTOM Série Entomologie Médicale et Parasitologie. 23(1):3–8.
Djohan V, Kaba D, Rayaissé JB, Dayo GK, Coulibaly B, Salou E, Dofini F, Kouadio ADMK, Menan H, Solano P. 2015. Detection and identification of pathogenic trypanosome species in tsetse flies along the Comoé River in Côte d’Ivoire. Parasite. 22:18. https://doi.org/10.1051/parasite/2015018. DOI: https://doi.org/10.1051/parasite/2015018
FAO & WHO. 2022. Vector control and the elimination of gambiense human African trypanosomiasis (HAT) - Joint FAO/WHO Virtual Expert Meeting – 5–6 October 2021. PAAT Meeting Report Series. No. 1. Rome. https://doi.org/10.4060/cc0178en. DOI: https://doi.org/10.4060/cc0178en
Farias IP, Ortí G, Sampaio I, Schneider H, Meyer A. 2001. The cytochrome b gene as a phylogenetic marker: the limits of resolution for analyzing relationships among Cichlid fishes. Journal of Molecular Evolution. 53(2):89–103. https://doi.org/10.1007/s002390010197. DOI: https://doi.org/10.1007/s002390010197
Fauret P, Dayo C, Rayaisse J-B, Pooda SH, Dofini F, Salano P, Calas B, Courtin F. 2015. Population dynamics, changes environmental and risk change trypanosome in the southwestof Burkina Faso from 2005 to 2014. Dynamiques Environnementales. 36(36):146–170. https://doi.org/10.4000/dynenviron.1015. DOI: https://doi.org/10.4000/dynenviron.1015
Franco JR, Simarro PP, Diarra A, Jannin J. 2014. Epidemiology of human African trypanosomiasis. Journal of Clinical Epidemiology. 6:257–275. DOI: https://doi.org/10.2147/CLEP.S39728
Gouteux JP. 1991. La raréfaction de tsé-tsé du groupe fusca en Afrique Centrale (Diptera, Glossinidae). Bulletin de la Sociét é Entomologique de France. 96(5):443–449. https://doi.org/10.3406/bsef.1991.17757. DOI: https://doi.org/10.3406/bsef.1991.17757
Gouteux JP, Laveissière C, Boreham PFL. 1982. Ecologie des glossines en secteur pré-forestier de Côte d’Ivoire: 2. Les préférences trophiques de Glossina palpalis s.l. Cahiers ORSTOM, Série Entomologie Médicale et Parasitologie. 20:3–18.
Gouteux JP, Laveissière C, Couret D. 1983. Ecologie des glossines en secteur pré-forestier de Côte d’Ivoire: Les lieux de reproduction Cahiers ORSTOM, série Entomologie Médicale et Parasitologie 21:3–12.
Hebert PDN, Cywinska A, Ball SL, Dewaard JR. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B, Biological Sciences 270: 313–321. DOI: https://doi.org/10.1098/rspb.2002.2218
Jamonneau V, Barnabé C, Koffi M, Sané B, Cuny G, Solano P. 2003. Identification of Trypanosoma brucei circulating in a sleeping sickness focus in Côte d’Ivoire: assessment of genotype selection by the isolation method. Infection, Genetics and Evolution. 3(2):143–149. https://doi.org/10.1016/S1567-1348(03)00069-8. DOI: https://doi.org/10.1016/S1567-1348(03)00069-8
Jamonneau V, Ravel S, Koffi M, Kaba D, Zeze DG, Ndri L, Sane B, Coulibaly B, Cuny G, Solano P. 2004. Mixed infections of trypanosomes in tsetse and pigs and their epidemiological significance in a sleeping sickness focus of Côte d’Ivoire. Parasitology. 129:693–702. https://doi.org/10.1017/S0031182004005876. DOI: https://doi.org/10.1017/S0031182004005876
Kaba D. 2006. Etude des glossines vectrices des Trypanosomoses Africaines et lutte antivectorielle au 43ème BIMA à Abidjan (Port-Bouët). Côte d’Ivoire: Université de Bouaké. 82 p.
Kaba D, Djohan D, Berté D, Ta BTD, Selby R, Kouadio KAM, Coulibaly B, Traoré G, Rayaisse JB, Fauret P, et al. 2021. Use of vector control to protect people from sleeping sickness in the focus of Bonon (Côte d’Ivoire). PLoS Neglected Tropical Diseases 15:e0009404. https://doi.org/10.1371/journal.pntd.0009404 DOI: https://doi.org/10.1371/journal.pntd.0009404
Kaba D, Koffi M, Kouakou L, N’Gouan EK, Djohan V, Courtin F, N’Djetchi MK, Coulibaly B, Adingra GP, Berté D, et al. 2023. Towards the sustainable elimination of gambiense human African trypanosomiasis in Côte d’Ivoire using an integrated approach. PLoS Neglected Tropical Diseases. 17:e0011514. https://doi.org/10.1371/journal.pntd.0011514. DOI: https://doi.org/10.1371/journal.pntd.0011514
Kazadi JM, Losson B, Kageruka P. 2000. Compétence vectorielle des mouches non ténérales de Glossina morsitans morsitans (Souche Mall) infectées par Trypanosoma (Nannomonas) congolense IL 1180. Bulletin de la Sociét é de Pathologie Exotique. 93:125–128.
Kébé BI, N’Guessan KF. 2003. Rapport de la mission de prospection du swollen shoot. 11–13 Septembre 2003. CNR.A. Côte d’Ivoire: Divo. 7 p.
Koffi M, N’Djetchi M, Ilboudo H, Kaba D, Coulibaly B, N’Gouan E, Kouakou L, Bucheton B, Solano P, Courtin F, et al. 2016. A targeted door-to-door strategy for sleeping sickness detection in low-prevalence settings in Côte d’Ivoire. Parasite. 23:51. https://doi.org/10.1051/parasite/2016059. DOI: https://doi.org/10.1051/parasite/2016059
Krouba GID, Ouattara AA, Kouakou ACA, Adopo ARI, Fauret P, Coulibaly B, Kaba D, Koffi YJJ, Assi Kaudhis PJ, Courtin F. 2018. Dynamiques de peuplement et modifications paysagères dans la zone rurale sud de la ville de Bonon entre 2000 et 2015 (Région de la Marahoué, Côte d’Ivoire). Tropicultura. 36(2):271–280.
Kohagne T, Mavoungou J, Fako H, Pamba R, Mbatchi B. 2013. Is there a suburban sleeping sickness in Libreville? African Health Sciences. 13(2):266–269. https://doi.org/10.4314/ahs.v13i2.10. DOI: https://doi.org/10.4314/ahs.v13i2.10
Lah EFC, Ahamad M, Haron MS, Ming HT. 2012. Establishment of a molecular tool for blood meal identification in Malaysia. Asian Pacific Journal of Tropical Biomedicine. 2(3):223–227. https://doi.org/10.1016/S2221-1691(12)60046-X. DOI: https://doi.org/10.1016/S2221-1691(12)60046-X
Laveissière C, Couret D, Staak C, Hervouët JP. 1985. Glossina palpalis et ses hôtes en secteur forestier de Côte d’Ivoire: relations avec l’épidémiologie de la trypanosomiase humaine. Cahiers ORSTOM, série Entomologie Médicale et Parasitologie 23: 297–303.
Laveissière C, Grébaut P. 1990. Recherches sur les pièges à glossines (Diptera : Glossinidae). Mise au point d’un modèle économique : le piège ‘Vavoua’. Tropical Medicine and Parasitology. 41:185–192.
Laveissière C, Grébaut P, Herder S, Penchenier L. 2000. Les glossines vectrices de la Trypanosomiase humaine. Montpellier: IRD Editions. 246 p.
Laveissière C. & Hervouët JP. 1981. Populations de glossines et systèmes d’occupation de l’espace : enquête entomologique dans la région de la Lobo (Côte d’Ivoire) février 1981. Cahiers ORSTOM, série Entomologie Médicale et Parasitologie 19:247–260.
Laveissière C, Hervouët JP. 1988. Epidémiologie et contrôle de la trypanosomiase humaine en Afrique de l’Ouest. ORSTOM Editions. 157 p.
Leak SGA. 1998. Tsetse biology and ecology: their role in the epidemiology and control of trypanosomosis. Wallingford, United Kingdom: CABI Publishing Editions. 568 p. https://doi.org/10.1079/9780851993003.0000. DOI: https://doi.org/10.1079/9780851993003.0000
Leak SGA, Ejigu D, Vreysen MJB. 2008. Collection of entomological baseline data for tsetse area-wide integrated pest management programmes. Rome: FAO. 205 p. https://openknowledge.fao.org/handle/20.500.14283/i0535e.
Lumbala C, Simarro PP, Cecchi G, Paone M, Franco JR, Kande Betu Ku Mesu V, Makabuza J, Diarra A, Chansy S, Priotto G, et al. 2015. Human African trypanosomiasis in the Democratic Republic of the Congo: disease distribution and risk. International Journal of Health Geographics. 14(1):20. https://doi.org/10.1186/s12942-015-0013-9. DOI: https://doi.org/10.1186/s12942-015-0013-9
Masiga DK, Smyth AJ, Hayes P, Bromidge TJ, Gibson WC. 1992. Sensitive detection of trypanosomes in tsetse flies by DNA amplification. International Journal for Parasitology . 22(7):909–918. https://doi.org/10.1016/0020-7519(92)90047-O. DOI: https://doi.org/10.1016/0020-7519(92)90047-O
Moser DR, Cook GA, Ochs DE, Bailey CP, McKane MR, Donelson JE. 1989. Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction. Parasitology. 99(1):57–66. https://doi.org/10.1017/S0031182000061023. DOI: https://doi.org/10.1017/S0031182000061023
N’Djetchi MK, Ilboudo H, Koffi M, Kaboré J, Kaboré JW, Kaba D, Courtin F, Coulibaly B, Fauret P, Kouakou L, et al. 2017. The study of trypanosome species circulating in domestic animals in two human African trypanosomiasis foci of Côte d’Ivoire identifies pigs and cattle as potential reservoirs of Trypanosoma brucei gambiense. PLoS Neglected Tropical Diseases. 11:e0005993. https://doi.org/10.1371/journal.pntd.0005993. DOI: https://doi.org/10.1371/journal.pntd.0005993
Nekpeni EB, Dagnogo M, Eouzan JP. 1989. Détermination de la limite géographique entre deux sous-espèces de glossines en Côte-d’Ivoire: glossina palpalis palpalis (Robineau-Desvoidy, 1830) et G. p. gambiensis (Vanderplank, 1949). Tropical Medicine and Parasitology. 40:12–15.
Njiokou F, Simo G, Mbida Mbida A, Truc P, Cuny G, Herder S. 2004. A study of host preference in tsetse flies using a modified heteroduplex PCR-based method. Acta Tropica. 91(2):117–120. https://doi.org/10.1016/j.actatropica.2004.03.006. DOI: https://doi.org/10.1016/j.actatropica.2004.03.006
Pollock JN, editor. 1982. Biologie, systématique et répartition des tsé-tsé. Vol. 1. Rome: FAO. 310 p. (Manuel de lutte contre la mouche tsé-tsé). https://openknowledge.fao.org/handle/20.500.14283/p5178f.
Ravel S, De Meeus T, Dujardin JP, Zézé DG, Gooding RH, Dusfour I, Sané B, Cuny G, Solano P. 2007. The tsetse fly Glossina palpalis palpalis is composed of several genetically differentiated small populations in the sleeping sickness focus of Bonon, Côte d’Ivoire. Infection, Genetics and Evolution. 7(1):116–125. https://doi.org/10.1016/j.meegid.2006.07.002. DOI: https://doi.org/10.1016/j.meegid.2006.07.002
Roubaud E. 1913. Les mouches tsé-tsé en Afrique Occidentale Française. Annales de Géographie. 22(126):427–450. https://doi.org/10.3406/geo.1913.8221. DOI: https://doi.org/10.3406/geo.1913.8221
Sané B, Garcia A, Fournet F, Laveissière C. 1999. Répartition des groupes d’âge de Glossina palpalis palpalis femelles dans les plantations et les talwegs en zone forestière de Côte d’Ivoire. Relation avec la prévalence de la maladie du sommeil. Bulletin de la Société de Pathologie Exotique. 92(3):210–212.
Sané B, Laveissière C, Méda HA. 2000. Répartition spatiale et préférences trophiques de Glossina palpalis palpalis dans le foyer forestier de Zoukougbeu (Cote d’Ivoire). Implications épidémiologiques. Parasite. 7(3):241–244. https://doi.org/10.1051/parasite/2000073241. DOI: https://doi.org/10.1051/parasite/2000073241
Simarro PP, Cecchi G, Paone M, Franco JR, Diarra A, Ruiz JA, Fèvre EM, Courtin F, Mattioli RC, Jannin JG. 2010. The Atlas of human African trypanosomiasis: a contribution to global mapping of neglected tropical diseases. International Journal of Health Geographics. 9(1):57. https://doi.org/10.1186/1476-072X-9-57. DOI: https://doi.org/10.1186/1476-072X-9-57
Simo G, Diabakana PM, Mesu VKBK, Manzambi EZ, Ollivier G, Asonganyi T, Cuny G, Grébaut P. 2006. Human African Trypanosomiasis Transmission, Kinshasa, Democratic Republic of Congo. Emerging Infectious Diseases. 12(12):1968–1970. https://doi.org/10.3201/eid1212.060516. DOI: https://doi.org/10.3201/eid1212.060516
Späth J. 2000. Feeding patterns of three sympatric tsetse species (Glossina spp.) (Diptera: Glossinidae) in the preforest zone of Côte d’Ivoire. Acta Tropica. 75(1):109–118. https://doi.org/10.1016/S0001-706X(99)00096-0. DOI: https://doi.org/10.1016/S0001-706X(99)00096-0
Steuber S, Abdel-Rady A, Clausen PH. 2005. PCR-RFLP analysis: a promising technique for host species identification of blood meals from tsetse flies (Diptera: Glossinidae). Parasitology Research. 97(3):247–254. https://doi.org/10.1007/s00436-005-1410-y. DOI: https://doi.org/10.1007/s00436-005-1410-y
Tano AS. 2012. Crise cacaoyère et stratégies des producteurs de la sous-préfecture de Méadji au Sud-Ouest ivoirien. Thèse de Doctorat, Université de Toulouse, 261 p.
Walsh PS, Metzger DA, Higuchi R. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 10(4):506–513.
WHO. 1986. Epidemiology and control of African trypanosomiasis: report of a WHO expert committee. Geneva: World Health Organization. 127 p. [meeting held in Geneva from 16 to 23 October 1985]].
WHO. 2013. Control and surveillance of human African trypanosomiasis: report of a WHO Expert Committee. Control and Surveillance of Human African Trypanosomiasis, Geneva, 22–26 April 2013. Geneva: World Health Organization. 237 p.
Yao Y, Green CH, Krüger WD, Sanou F, Toure F. 1997. Etude de l’infection trypanosomienne de Glossina longipalpis Wiedemann, 1840 (Diptera: Glossinidae) et de ses variations en secteur préforestier de Côte d’Ivoire. Société Française d’Ethnopharmacologie 10263:266–270.
Yoni W, Bila C, Bouyer J, Desquesnes M, Idrissa Kaboré I. 2005. La dissection des glossines ou mouches tsé-tsé. Santé animale en Afrique de l’Ouest. Fiche technique 23, 12 pp.
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